Author Archives: mwaring708

2013 Salary Survey

Posted on May 8, 2014 by mwaring708

–Thanks to Stephen Kwok at the Tufts Laser Cytometry Flow Core at Tufts Medical School for conducting a flow cytometry salary survey last year, the results were posted to the Purdue list but have since been removed, so we are … Continue reading →

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One of these things is not like the others…

Posted on January 16, 2014 by mwaring708

For those of you that grew up with Sesame Street: Three of these things belong together Three of these things are kind of the same Can you guess which one of these doesn’t belong here? Now it’s time to play … Continue reading →

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A clone by any other name…

Posted on June 5, 2013 by mwaring708
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Bee-ah-core or Bye-ah-core?

Posted on April 26, 2013 by mwaring708

We have a Biacore 3000 from GE Healthcare, which uses Surface Plasmon Resonance technology (SPR) to perform label-free measurements of protein interactions, as well as detect differences in concentrations of solutions.  This technology can determine binding kinetics and protein interactions … Continue reading →

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FCS Files

Posted on March 26, 2013 by mwaring708
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Comp Comp

Posted on March 11, 2013 by mwaring708

Compensation Comparison I often hear of people looking at a compensation matrix and deciding if it is “good” or “bad”.  The actual values in themselves don’t give enough information to make this determination.  Voltages can be set to give you … Continue reading →

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You have chosen… poorly

Posted on February 26, 2013 by mwaring708

It was brought to my attention that the filter on our 3 laser LSR2 might not be optimal for the AmCyan channel (490/40 bandpass, also used for Horizon V500, CFP, Aqua Live/Dead dye, and Q525)–it was centered on the peak … Continue reading →

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Staying focused….

Posted on January 23, 2013 by mwaring708

Just a quick post since we are in the midst of relocating to a new facility…

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As bright or brighter…

Posted on January 15, 2013 by mwaring708

So the earlier posts were a lead-in to this one, which I hope will clarify this important issue in compensation.  When calculating compensation, it is important to make sure that your single stained control is as bright or brighter than … Continue reading →

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Compensation caution: matching beads

Posted on January 4, 2013 by mwaring708

Other platforms may be different, but with BD’s Diva software you can only collect one “unstained” compensation control, so if it is necessary to mix what type of compensation particle you are using (cells for PI or DAPI, Arc beads … Continue reading →

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