Author Archives: mwaring708

One of these things is not like the others…

For those of you that grew up with Sesame Street: Three of these things belong together Three of these things are kind of the same Can you guess which one of these doesn’t belong here? Now it’s time to play … Continue reading

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A clone by any other name…

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Bee-ah-core or Bye-ah-core?

We have a Biacore 3000 from GE Healthcare, which uses Surface Plasmon Resonance technology (SPR) to perform label-free measurements of protein interactions, as well as detect differences in concentrations of solutions.  This technology can determine binding kinetics and protein interactions … Continue reading

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FCS Files

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Comp Comp

Compensation Comparison I often hear of people looking at a compensation matrix and deciding if it is “good” or “bad”.  The actual values in themselves don’t give enough information to make this determination.  Voltages can be set to give you … Continue reading

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You have chosen… poorly

It was brought to my attention that the filter on our 3 laser LSR2 might not be optimal for the AmCyan channel (490/40 bandpass, also used for Horizon V500, CFP, Aqua Live/Dead dye, and Q525)–it was centered on the peak … Continue reading

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Staying focused….

Just a quick post since we are in the midst of relocating to a new facility…

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As bright or brighter…

So the earlier posts were a lead-in to this one, which I hope will clarify this important issue in compensation.  When calculating compensation, it is important to make sure that your single stained control is as bright or brighter than … Continue reading

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Compensation caution: matching beads

Other platforms may be different, but with BD’s Diva software you can only collect one “unstained” compensation control, so if it is necessary to mix what type of compensation particle you are using (cells for PI or DAPI, Arc beads … Continue reading

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Full Disclosure: I did not receive any Compensation for this post…

In flow cytometry it seems the biggest source of confusion and angst is compensation.  As a first post, I’m going to re-publish a post I did elsewhere. Compensation is somewhat tricky if you don’t understand the process. There are lots … Continue reading

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