Author Archives: mwaring708

NECyto 2014 and some updates

The New England Cytometry Users Group would like to announce the date for our annual Fall meeting.  It will be on October 2nd  from 8:00 am to 5 pm at the beautiful Starr Center at the Schepens Eye Research Institute in … Continue reading

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2013 Salary Survey

–Thanks to Stephen Kwok at the Tufts Laser Cytometry Flow Core at Tufts Medical School for conducting a flow cytometry salary survey last year, the results were posted to the Purdue list but have since been removed, so we are … Continue reading

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One of these things is not like the others…

For those of you that grew up with Sesame Street: Three of these things belong together Three of these things are kind of the same Can you guess which one of these doesn’t belong here? Now it’s time to play … Continue reading

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A clone by any other name…

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Bee-ah-core or Bye-ah-core?

We have a Biacore 3000 from GE Healthcare, which uses Surface Plasmon Resonance technology (SPR) to perform label-free measurements of protein interactions, as well as detect differences in concentrations of solutions.  This technology can determine binding kinetics and protein interactions … Continue reading

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FCS Files

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Comp Comp

Compensation Comparison I often hear of people looking at a compensation matrix and deciding if it is “good” or “bad”.  The actual values in themselves don’t give enough information to make this determination.  Voltages can be set to give you … Continue reading

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You have chosen… poorly

It was brought to my attention that the filter on our 3 laser LSR2 might not be optimal for the AmCyan channel (490/40 bandpass, also used for Horizon V500, CFP, Aqua Live/Dead dye, and Q525)–it was centered on the peak … Continue reading

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Staying focused….

Just a quick post since we are in the midst of relocating to a new facility…

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As bright or brighter…

So the earlier posts were a lead-in to this one, which I hope will clarify this important issue in compensation.  When calculating compensation, it is important to make sure that your single stained control is as bright or brighter than … Continue reading

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